Pyruvate Oxidation by Extracts of Tetrahymena pyriformis
نویسندگان
چکیده
منابع مشابه
A NEW BIOLOGICAL SCREENING SYSTEM FOR LOCAL ANAESTHETICS BY INHIBITION MOBILITY OF TETRAHYMENA PYRIFORMIS
An alternative in vitro approach to drug screening has been the use of human cell cultures for antiviral agents and microbial cell cultures for the assessment of the carcinogenic potential of selected compounds. A number of protozoan species have been also used as drug screens for anti-protozoal agents. The ciliated protozoan Tetrahymena pyriformis species has been widely utilised as a dru...
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The cellular site of synthesis of ribosomal RNA in Tetrahymena pyriformis was studied by analyzing the purified nuclear and cytoplasmic RNA from cells pulse labeled with uridine-(3)H. The results of studies using zonal centrifugation in sucrose density gradients show that the ribosomal RNA is synthesized in the nucleus as a large precursor molecule sedimenting at 35S. The 35S molecule undergoes...
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A method has been devised to fractionate cells of Tetrahymena pyriformis, yielding pure or highly enriched preparations of cilia, cilia-associated soluble material, pellicles, mitochondria, microsomes, and postmicrosomal supernatant. The method prevents the destructive action of lipolytic enzymes commonly associated with this organism. Analysis of the membrane lipids of these fractions reveals ...
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Cyclopropane fatty acids are readily metabolized by whole cells of Tetrahymena pyriformis when the fatty acids are presented either as components of intact Escherichia coli cells or as free fatty acids added to the medium. cis-ll,lZ[methylene-14C]Methyleneoctadecanoic acid was degraded with the production of 14COz by whole T. pyriformis cells or a particulate fraction derived from them. In the ...
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In-depth quantitative visualization studies are required to understand the flow induced by swimming micro-organisms and find potential applications. The present study visualized the flow induced by Tetrahymena pyriformis of size 45–50 lm, which swam freely and via stimulation by galvanotaxis in a PDMS micro-chamber using a micro-particle image velocimetry system. The results showed that the max...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1954
ISSN: 0022-1287
DOI: 10.1099/00221287-11-2-300